摘要
采用高效液相色谱法，以电化学检测测定双黄连粉针中黄芩甙和黄芩素的含量。流动相０１ｍｏｌ／Ｌ磷酸二氢钾乙酸四氢呋喃（１０００∶１００∶１９０），色谱柱ＥＲＣＯＤＳ１１６１（６ｍｍ×１００ｍｍ），检测电压＋６５０ｍＶ，柱温５５℃，流速０８ｍｌ／ｍｉｎ。结果：本法的最小检测限为２５ｎｇ和５０ｎｇ，平均回收率为１００１％和９９７％，重现性好。本法测定快速、简便、准确，适用于含黄芩甙和黄芩素的中药材和制剂的含量测定。
The contents of baicalin and baicalein in Shuanghuanglian Powdery Injection have been determined by the method of HPLC ECD under following conditions: column ERC ODS 1161,6mm×100mm; temperature 55℃; mobile phase 0.1 mol/L KH 2PO 4,acetic acid and tetrahydrofuran (1000∶100∶190); flow rate 0.8ml/min;detector voltage +650mV.The result shows the limit of detection for baicalin and baicalein to be 25ng and 50ng respectively,with an average recovery of 100.1% and 99.7%respectively.Highly reproducible,rapid,simple,convenient and accurate,this method is good for the content determination of baicalin and baicalein in Chinese medicinal herbs and preparations.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1997年第10期604-605,共2页
China Journal of Chinese Materia Medica
关键词
高效液相色谱
黄芩甙
黄芩素
双黄连粉针
HPLC ECD method
baicalin
baicalein
Shuanghuanglian Powdery Injection
