摘要
用MS／MS作为HPLC的检测器，采用多反应监测（MRM）扫描方式，建立了HPLC—MS／MS测定藜蒿中绿原酸含量的方法。该方法的线性范围为2．0—250μg／L。检出限为0．5μg/L。通过样品的不同加标试验，回收率为96％～98％，精密度RSD（n=5）为1．3％～2．2％。实际样品分析表明，藜蒿茎中绿原酸的含量为11．8mg／g。本方法选择性高、灵敏、快速，也适用于其它复杂体系中绿原酸含量的快速测定。
A method was developed for the determination of chlorogenic acid in Artemisia Selergensis Turcz extracts by HPLC - MS/MS, using tandem mass spectrometer as a detector. Under the optimized conditions, a good linear range was obtained from 2.0 to 250 μg/L, and the detection limit was 0.5 μg/L. The recoveries of the analyte at the spiking concentrations of 4.0, 20.0, and 100 μg/L were 96% , 96% , and 98% , respectively, with relative standard deviations of 2.2% , 1.8% , and 1.3% （ n = 5 ）. Analysis of real samples showed that the content of chlorogenic acid in Artemisia Selergensis Turcz was 11.8 mg/g. The proposed method is also suitable for rapid determination of chlorogenic acid in complex matrices.
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第3期89-91,共3页
Journal of Instrumental Analysis
基金
江西省教育厅科技计划项目(赣教技字[2005]166号)
南昌大学食品科学教育部重点实验室开放基金资助项目(NCU200408)
关键词
藜蒿
串联质谱
绿原酸
多反应监测
Artemisia Selergensis Turcz
Tandem mass spectrometry
Chlorogenic acid
Multiple reaction monitoring
