摘要
目的：建立狭叶薰衣草药材中迷迭香酸的含量测定方法，为其质量控制提供科学依据。方法采用 HPLC法测定，色谱条件：Phenomenex ODS-A色谱柱（250 mm ×4．6 mm ，5μm ）；流动相为乙腈和2 mL · L -1磷酸二元梯度洗脱；流速：1．0 mL · min-1；柱温：30℃；检测波长为350 nm。结果测定了10批不同产地市售的狭叶薰衣草药材，迷迭香酸的含量在0．04％～0.33％之间。结论该方法操作简便，重复性好，可用于规范和控制狭叶薰衣草药材的质量。
Objective To establish a determination method for rosmarinic acid in Lavandula angustifolia by HPLC .Methods The HPLC separation was performed on a Phenomenex ODS-A column（250 mm × 4 .6 mm ,5μm） in a gradient elute mode with a mix-ture consisting of acetonitrile and 2 mL · L -1 phosphate acid solution at 30 ℃ .The flow rate was 1 .0 mL · min-1 and the detec-tion wavelength was set at 350 nm .Results The contents of rosmarinic acid in 10 samples of L .angustifolia were in the rangs of 0 .04%-0 .33% .Conclusion The method is simple ,accurate and reproducible ,and can be used for the quality control of L .angus-ti f olia .
出处
《西北药学杂志》
CAS
2014年第3期230-232,共3页
Northwest Pharmaceutical Journal
基金
新疆药物研究所科研基金项目(编号:XYSS-2012-7)
