摘要
以薰衣草叶片为外植体,建立了薰衣草高频植株再生系统,为薰衣草规模化快繁和遗传转化体系的建立奠定了基础。以6-BA,NAA,IBA,Ce(NO3)2,Na2MoO4及蔗糖为实验因子,芽分化率为测定指标,通过L18(61×36)正交实验,筛选了薰衣草芽分化的最佳组合:MS培养基中含有6-BA2.0mg/L,NAA0.5mg/L,IBA0.15mg/L,Ce(NO3)210mg/L,Na2MoO40.5mg/L及蔗糖15g/L。通过L9(34)正交实验,筛选了1种宜于薰衣草生根的理想培养基,即MS+IAA0.5mg/L+NAA0.1mg/L+蔗糖15g/L+活性炭1.0g/L。
A high frequency plant regeneration system for Lavandula angustifolia was established to lay a foundation for massive micropropagation and genetic transformation of the plant. The results of an orthogonal experiment showed that the optimum medium for shoot differentiation was MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L + IBA 0.15 mg/L + Ce(NO_3)_2 10 mg/L + Na_2MoO_4 0.5 mg/L + sucrose 15 g/L and the optimum rooting medium was MS + IAA 0.5 mg/L + NAA 0.1 mg/L + sucrose 15 g/L + active carbon 1.0 g/L.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2005年第3期344-349,共6页
Journal of Southwest Agricultural University
基金
中国科学院西部行政高新技术资助项目(KGCX2-SW-506)
