摘要
目的 :建立香薷药材中香芹酚和百里香酚的含量测定方法。方法 :用高效液相色谱法 ,95 %乙醇超声提取 ,色谱柱InertsilODS 3,流动相甲醇 水 冰醋酸 (6 0∶4 0∶2 ) ,检测波长 2 74nm。结果 :香芹酚在 0 2 3～ 2 15 μg (r=0 9999) ,百里香酚在 0 39～ 2 36 μg (r =0 9999)有良好的线性关系 ;平均回收率分别为香芹酚 99 9% (RSD1 4 % ) ,百里香酚 98 6 % (RSD 1 3% ) ,重复性的RSD分别为香芹酚 1 1% ,百里香酚 1 6 %。结论 :该方法可靠、便捷 ,可以作为控制香薷药材质量的方法。
Objective: To establish a quantitative method o f determination of carvacrol and thymol in Mosla chinensis. Method: The sample was extracted with 95% ethanol, ODS column was used with met hanol-water-acetic acid (60∶40∶2) as mibile phase. The detection wavelength was set at 274 nm. Result: The linearities of carvacrol and thymol were respectively in the range of 0.23～2.15 μg (r=0.999 9) and 0.39～2.36 μg (r=0.999 9); the average recoveries were 99.9% (RSD 1.4%) and 98.6% (RSD 1.3%); the RSD of repeatability were 1.1% and 1.6% . Conclusion: The mothod is reliable, and can be used for qu ality control of M. chinensis. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2004年第11期1030-1032,共3页
China Journal of Chinese Materia Medica
基金
中央级科研院所科技基础性工作专项资金
