摘要
目的:探讨右归丸对实验性自身免疫性脑脊髓炎小鼠(experimental autoimmune encephalomyelitis,EAE)IL-23/IL-17炎性轴的影响。方法:建立髓鞘少突胶质细胞糖蛋白35-55(myelin oligodendrocyte glycoprotein 35-55,MOG35-55)诱导C57BL/6小鼠EAE模型,随机分为模型组、泼尼松组,右归丸组,并设正常对照组。观察小鼠的发病情况、神经功能评分及体重变化,HE染色观察CNS病理损伤程度,实时荧光定量PCR法检测小鼠脑组织IL-23 p19、IL-17 mRNA表达。结果:与正常对照组比较,模型组脑组织IL-23 p19、IL-17mRNA表达显著升高,泼尼松及右归丸组小鼠脑组织IL-23 p19、IL-17的mRNA表达比模型组低(P<0.01)。结论:右归丸能够抑制IL-23/IL-17炎性轴而起到抗炎治疗作用。
Objective：To investigate the effects of Yougui Pill on IL -23/IL - 17 axis of mice with acute experimental autoim- mune encephalomyelitis （EAE）. Methods ：EAE model was induced in C57BL/6 mice,and was treated with Yougui Pill （Yougui Pill group） ,prednisone （prednisone group） and saline（ EAE group）. Normal mice were selected as control group. Levels of IL- 23p19 ,IL- 17 mRNA expression in brain tissue were detected by real time- PCR. Results：IL- 23p19, IL- 17 mRNA levels in brain tissue were significantly decreased in Yougui Pill and prednisone treated EAE mice than those in EAE mice （P 〈 0.01 ）. Conclusion：Yougui Pill may exert anti -inflammatory effect through inhibiting IL- 23/IL- 17 axis.
出处
《辽宁中医杂志》
CAS
2013年第10期2149-2151,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(30901909)
高等学校博士学科点专项科研基金新教师基金项目(200800261037)
