摘要
目的探讨重症急性胰腺炎(SAP)大鼠血清乙酰胆碱酯酶(TRUE CHOLINE ESTERASE,TCHE)和乙酰胆碱转移酶(CHOLINE ACETYLTRANSFERASE,CHAT)的变化及其与IL-6和TNF-Α相关性以及柴芩承气汤(CHAI QIN CHENGQI DECOCTION,CQCQD)对其的影响。方法将30只SD大鼠随机分成3组(假模手术型组、SAP组和CQCQD治疗组),每组10只。检测各组血生化指标、淀粉酶、TNF-Α、IL-6、TCHE及CHAT。结果SAP组较假手术模型组血清IL-6、TNF-Α及TCHE升高(P<0.01),血清CHAT降低(P<0.01);血清IL-6与TCHE呈正相关(R=0.95,P=0.000),与CHAT呈负相关(R=-0.91,P=0.000);TNF-Α与TCHE呈正相关(R=0.93,P=0.000),与CHAT呈负相关(R=-0.95,P=0.004)。CQCQD治疗组血清IL-6、TNF-Α较SAP组低(P<0.01),血清CHAT较SAP组高(P<0.01)。假手术模型组、CQCQD治疗组和SAP组WBC、ALT、AST及LDH依次升高(P<0.01)。结论胆碱能抗炎通路在SAP大鼠的病理生理过程中起着重要作用。柴芩承气汤可通过影响其功能,减轻全身炎性反应综合征,减少脏器功能受损。
Objective To test the change of true choline esterase （TChE） and cholineacetyltransferase （CHAT） and their correlation with interleukin-6 （IL-6） and Tumor Necrosis Factor-α （TNF-α）, and the impact of Chai Qin Cheng Qi Decoction （CQCQD） on cholinergic anti-inflammatory pathway in rats with severe acute pancreatitis （SAP）. Methods Thirty SD rats were randomly divided into three groups： control group （pseudooperated）, SAP group and CQCQD-treated group, each with 10 rats. Blood samples were taken six hours after injection of testing agents for biochemical test, which included the test of amylase, TNF-α, IL-6, TChE, and CHAT. Results The rats in SAP group had higher levels of serum IL-6, TNF-αand TChE and lower levels of serum ChAT than those in control group （P〈0.05）. The serum IL-6 was positively correlated with TChE （r= 0. 95, P=0. 000） and negatively correlated with ChAT （r=-0. 91, P=0. 000）. The TNF-α was also positively correlated with TChE （r=0. 93, P=0. 000） and negatively correlated with ChAT （r=-0. 95, P=0. 004）. The rats in CQCQD-treated group had lower levels of serum IL-6, TNF-α and TChE and higher levels of serum ChAT than those in SAP group （P 〈 0. 01）. The increase of white blood cell, alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase was observed in the control group first and followed by the CQCQD-treated group and SAP group sequentially （P〈0. 05）. Conclusions Cholinerglc antiinflammatory pathway plays an important role in the pathological changes of SAP in rats. CQCQD can relieve the systemic inflammatory response syndrome and reduce the functional damage of organs through interference on the cholinergic antiinflammatory pathway. More studies are needed to reveal the mechanism of such impact.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第1期66-68,共3页
Journal of Sichuan University(Medical Sciences)
关键词
重症急性胰腺炎
乙酰胆碱酯酶
乙酰胆碱转移酶
胆碱能抗炎通路
柴芩承气汤
Acute pancreatitis True choline esterase Choline acetyltransferase Cholinerglc anti-inflammatory pathway Chai Qin Cheng Qi Decoction
