摘要
通过镜检花粉各发育时期及其与花蕾、花药形态的相关性,探讨了花生基因型、液体培养基及附加激素浓度对愈伤组织诱导率的影响。结果表明,当花蕾纵径长为2.48～2.96mm,小孢子基本处于四分体时期;长度在3.26～4.16mm时,以单核期为主;5.38～9.9mm,为双核期。在小孢子单核靠边期取材诱导率最高,达9.38%。本实验所用的六个花生品种中,四粒红的单蕾产胚数最高,达到0.1个胚/蕾(MS培养基)和0.05个胚/蕾(NLN培养基);在附加4mg/L 2,4-D,3mg/L 6-BA和0.3mg/L NAA改良的MS液体培养基上且当小孢子密度为2.5×105个/mL时,花生品种四粒红的愈伤组织诱导率最高,为12.5%。
The relevance of pollen development stages,shape of bud and anther was discussed,and the effect of genotypes of isolated microspore,different kinds of liquid medium as well as additional hormone concentration on the callus was studied.The results showed that,when the longitudinal diameter of bud were 2.48~2.96mm,3.26~4.16mm and 5.38~9.9mm,development stages of pollen were mostly at tetrad,mononuclear period and uninucleate respectively.At late-uninucleate,the induction rate was the highest,at 9.38%.Six varieties of peanut were used in this experiment,the average embryo yield of Silihong was the highest,with 0.1 embryo per bud（MS medium） and 0.05 embryo per bud（NLN medium）.On MS medium of 4mg/L 2,4-D,3mg/L 6-BA and 0.3mg/L NAA,with 2.5×105/mL concentration of microspore,the induction rate of Silihong was the highest at 12.5%.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2012年第6期592-597,共6页
Chinese Journal of Oil Crop Sciences
基金
现代农业产业技术体系建设专项基金(CARS-14)
河北省应用基础研究计划重点基础研究项目(10960120D)
河北省自然科学基金(C2009000591)
关键词
花生
小孢子
液体悬浮培养
愈伤组织
激素组合
Peanut
Microspore
Fluid suspension culture
Callus
Hormonal combination
