摘要
综述了不同的培养基激素和碳源对瓜类花药组织培养的影响,以期为瓜类花药组培工作提供参考依据,并提出了以后的研究方向。结果发现,适当提高2,4-D含量可提高花药培养中愈伤组织诱导率:适当提高6-BA或KT浓度,可提高直接增殖率分化率;当6-BA高浓度时能显著促进不定芽的形成;当KT浓度高时,能促进根的分化并且对芽的分化表现出抑制作用;而生长素和细胞分裂素在植物不同生长时期的需求配比有所变化,激素在不同组织培养中的协同作用也对瓜类花药组织培养有着显著影响。碳源一般在6%～12%变化范围即可满足瓜类植物营养需求。
Anther culture is an important method among the breeding technologies of eueurbitaeeous crops. The effect of hormone and carbon sources used in different medium is set forth. The review is a consult for the anther euhure, and bring forward further research direction. Analysis reveals that proper increasing of concentration of 2,4-D or 6-BA/KT, the inducement rate or multiplication rate be heightened,respectively. The high concentration of 6-BA or KT accelerate the formation of adventitious bud or root, respectively. The proportion of different hormones in the same medium will co- operate with each other. The interaction of them should not be ignored. Carbon sources will meet the plant nutrition need with a range between 6% and 12%.
出处
《长江蔬菜》
北大核心
2008年第07X期5-9,共5页
Journal of Changjiang Vegetables
基金
贵州大学SRT项目资助(贵大SRT字2007059号)
关键词
花粉
培养基
组织培养
激素
碳源
Anther culture
Culture medium
Hormone
Carbon sources
