摘要
选用对小孢子培养反应较好的白菜型油菜地方品种 (茅山种 )为供体材料 ,研究了换培养液和秋水仙碱直接处理分离小孢子对胚产量的影响 ,并分析了秋水仙碱直接处理小孢子所获再生植株的染色体倍性水平。结果表明 ,分离小孢子在 1 0 %蔗糖浓度的NLN 1 0培养基上培养 2d后换成新鲜培养液 ,能显著提高出胚产量 ,比不换培养液的胚产量高1 5倍。更换培养液还能明显改善胚状体质量。用 0 8mg L秋水仙碱处理刚分离的小孢子 2d ,能显著提高出胚产量 ,并能提高再生植株二倍体率。而过高秋水仙碱浓度 (4～ 2 0mg L)未能提高出胚产量 ,且对胚状体萌发和小植株再生产生不利影响。
Using Brassica rapa syn B campestris ssp chinensis cv Maoshan (a local variety) which had good response to microspore culture as donor plant, the experiment was carried out to evaluate the effects of medium renovation and direct colchicine application on the embryogenesis of isolated microspores The chromosome ploidy level in regenerated plants derived from direct colchicine treatment of microspores was also analyzed in the present experiment The results indicated that the change of liquid NLN 10 medium with 10 % sucrose after 2 d initiation showed a significant increase in embryogenesis (by a 1 5 fold), compared to non medium renovation control In addition, the quality of embryos was greatly improved through medium change Direct colchicine treatment of isolated microspores with the concentration of 0 8 mg/L for 2 d produced the most embryos, and the highest doubling efficiency of 74 3% was obtained Whereas higher colchicine concentrations of 4～20 mg/L were unable to improve embryogenesis, and the adverse effects on embryo germination and plant regeneration were also observed
出处
《作物学报》
CAS
CSCD
北大核心
2004年第1期78-81,共4页
Acta Agronomica Sinica
基金
教育部留学回国人员科研启动基金
中瑞政府间合作项目 (C0 8)
浙江省重点科技项目 ( 0 2 110 2 10 9)资助
