摘要
在１／２ＮＬＮ培养基、３２．５℃、２４ｈ热激处理下，对３份绿菜花品种进行游离小孢子培养，结果表明，悬浮培养液中双核期小孢子比例对胚胎发生影响很大。品种的基因型不同，适宜的双核期小孢子比例也不同，‘ｂｃ－０’、‘Ｇａｌａｘｙ’在双核期小孢子比例分别为１５．３８％和１３．６０％时，出胚率最高；‘ｂｃ－４’则在５０．７％时出胚率最高，达３７．０８胚／蕾。
Isolated microspores of 3 genotypes of broccoli were cultured in 1/ 2NLN medium, pretreated at 32.5℃ for 24h. Results showed that the binucleate pollen ratio affected embryogenesis significantly. Different genotypes had different proper binucleate pollen ratio. For ‘bc-0’ and ‘Galaxy’ , 15.38% and 13.60% binucleate pollen ratio gave the highest embryo yield; For ‘bc-4’ , the highest embryo yield was obtained with 50.7% binucleate pollen ratio and it was 37.08 embryo per bud.
出处
《园艺学报》
CAS
CSCD
北大核心
1998年第2期201-202,共2页
Acta Horticulturae Sinica
