摘要
以8个红花品种(系)为试验材料,分析小孢子不同发育时期的细胞学特征,研究小孢子发育时期与花器官形态特征的关系,为采用花器官形态特征判断小孢子发育时期开展红花小孢子液体培养提供依据。结果表明,相同播期条件下,不同红花品种(系)因生育期进程不同导致小孢子发育时期不同,但小孢子沿雄配子发育的进程相同,均经历了小孢子母细胞时期、二分体时期、四分体时期、单核早中期、单核靠边期和双核期,且各发育时期细胞学特征明显。同一品种(系)单果球管状花的小孢子发育时期不同。8个红花品种(系)单核早中期的果球直径为0.90~1.70 cm、管状花长度为0.37~0.45 cm;单核靠边期的果球直径为1.00~1.96 cm、管状花长度为0.45~0.50 cm。8个红花品种(系)小孢子单核早中期、单核靠边期、双核期果球直径在大部分品种(系)间差异达到极显著水平,而管状花长度则表现为大部分品种(系)间差异未达到极显著水平。因此,在红花田间取材操作过程中,可根据管状花长度判断小孢子发育时期,再选择直径一致的果球取材。
Eight safflower varieties(lines)were used as experiment materials,cytological characteristics at different microspore development periods were investigated,and relationship between the microspore development period and floral organ morphology was studied,which would provide evidences for identifing microspore development periods based on floral organ morphology of safflower. The results showed that the growth and development process of different safflower varieties(lines)under the same sowing time was different,resulting in different microspore development period,but microspore development stages were uniform including microspore mother cell,dyad stage,tetrad stage,early-and mid-uninucleate stage,late-uninucleate stage and binucleate stage. Microspore development period of tubular flowers for the same fruit was different. Fruit diameter of eight safflower varieties(lines)was0. 90—1. 70 cm,and length of tubular flower was 0. 37—0. 45 cm at early-and mid-uninucleate stage.Fruit diameter of eight safflower varieties(lines)was 1. 00—1. 96 cm,and tubular flower length was0. 45—0. 50 cm at late-ninucleate stage. There were extremely significant differences in fruit diameter among most of the eight safflower varieties(lines)in the early-and mid-uninucleate stage,late-uninucleate stage and binucleate stage,but no extremely significant differences in length of tubular flower among most of the eight safflower varieties(lines). Therefore,according to tubular flower length,the development period of microspore will be judged in practice,and then the same fruit diameter will be selected.
作者
许兰杰
梁慧珍
余永亮
杨青
杨红旗
谭政委
董薇
李磊
安素妨
鲁丹丹
XU Lanjie;LIANG Huizhen;YU Yongliang;YANG Qing;YANG Hongqi;TAN Zhengwei;DONG Wei;LI Lei;AN Sufang;LU Dandan(Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)
出处
《河南农业科学》
北大核心
2021年第11期55-61,共7页
Journal of Henan Agricultural Sciences
基金
国家现代农业产业技术体系建设专项(CARS-21)
河南省农科院创新创意项目(2020CX03,2020CX19)
河南省农科院优秀青年基金项目(2020YQ05,2020YQ28)
河南省农科院自主创新专项(2021ZC72)
河南省农科院新兴学科发展专项(2020XK07,2021XK03)
河南省科技攻关项目(202102110297)。
关键词
红花
小孢子
细胞学特征
花器官形态
Safflower
Microspore
Cytological characteristics
Floral organ morphology
