摘要
以蝉蜕、蝇蛆壳两种培养基培养球孢白僵菌不同世代菌株 ,分析其类枯草杆菌蛋白酶(Pr1)和总胞外蛋白酶的产生水平及其与菌株毒力的相关性。结果表明 ,菌株的 Pr1产生水平达到最高的时间较总胞外蛋白酶早 1～ 2天。在蝉蜕培养基中 ,菌株 Pr1产生水平较高 ,而总胞外蛋白酶产生水平大大低于蝇蛆壳培养基中的产生水平。随着世代数的增加 ,Pr1和总胞外蛋白酶产生水平及对家蚕的毒力都明显下降。不同世代菌株在蝉蜕和蝇蛆壳培养基上的 Pr1产生水平与其毒力的相关系数分别为 0 .92和 0 .89,高于总胞外蛋白酶与其毒力的相关系数(0 .84和 0 .6 7)。因此认为在总胞外蛋白酶中 ,Pr1是影响菌株主要毒力因子之一 。
The production of subtilisin like protease (Prl) and total extracellular protease from different subcultures of Beauveria bassiana was investigated in cicada exuviae and maggot cuticle liquid culture. The relationship between protease production and virulence to Bombyx mori was compared. The results showed that Prl produced earlier than that of total extracellular protease, specially in cicada exuviae culture. The proteases production by different subcultures was significantly related to their virulence toB.mori. The relationship between Prl production with virulence was higher than that of total extracellular protease.
出处
《中国生物防治》
CSCD
2000年第2期61-64,共4页
Chinese Journal of Biological Control
基金
国家自然科学基金
重庆市应用基础研究资助项目
关键词
球孢白僵菌
类枯草杆菌蛋白酶
胞外蛋白酶
毒力
Beauveria bassiana
subtilisin like protease
total extracellular protease
virulence
