摘要
为筛选桃小食心虫高致病性菌株,以在山西省自然感病的桃小食心虫越冬幼虫上分离获得的5株病原真菌为材料,研究各菌株感染桃小食心虫幼虫的症状、对寄主的致死率,以及与菌株毒力相关的胞外类枯草杆菌蛋白酶、几丁质酶和脂肪酶的活性变化,并评价其生物防治潜力。5个菌株在寄主上的感病症状和致死率有显著差异,感病后10天,球孢白僵菌BbTST05对寄主的致死率最高,为89.3%,其类枯草杆菌蛋白酶、几丁质酶和脂肪酶3种酶活性均最大;粉棒束孢IfTSL02对寄主的致死率及其蛋白酶、几丁质酶活性次之;尖孢镰孢菌的FoTSL01、FoTSL03和FoTSL04菌株对桃小食心虫的致死率均低于61%,3种胞外酶的活性也较低。因此,球孢白僵菌BbTST05和粉棒束孢IfTSL02可用于桃小食心虫的防治。
In order to select some high pathogenicity strains in the entomogenous fungi as biological con trol agent against Carposina sasakii （Matsumura）, a destructive pest in fruit orchards, the five funga strains in three species were studied. The five strains were originally isolated from the natural diseasec larvae of C. sasakii infesting in Shanxi Province, China. Their virulence was evaluated by comparing th~ diseased symptom, infected insect mortality, and the activities of the subtilisin-like protease, chitinase, and fipase of the strains. The results showed significant difference in the host insect＇ s diseased sympton and mortality among the five different strains. After 10 days post infected, the highest host mortalit 89.3% were found in the experimental section of the strain Beauveria bassiana BbTST05, and mean while, the activities of subtilisin-like protease, chitinase, and lipase of the strain all were the highest it the five strains. Thus, it was the highest virulence strain against C. sasakii. The strain Isaria farinosa If- TSL02 caused higher host mortality and showed higher activities of subtilisin-like protease and chitinase. However, the host mortalities caused by the three strains FoTSL01, FoTSL03, and FoTSL04 of Fusariu~ oxysporum were all below 61% , and the activities of the three extracellular enzymes of these three strain were lower. Therefore, the two strains BbTST05 and IITSL02 could be used to control C. sasakii.
出处
《植物保护学报》
CAS
CSCD
北大核心
2012年第6期549-555,共7页
Journal of Plant Protection
基金
国家公益性行业(农业)科研专项(200803006)
关键词
桃小食心虫
昆虫病原真菌
毒力
胞外酶
Carposina sasakii
entomopathogenic fungus
virulence
extracellular enzyme
