摘要
目的:建立HPLC法同时测定不同产地莪术中莪术二酮、莪术醇、吉玛酮、呋喃二烯、β-榄香烯等5种倍半萜类成分的含量。方法:采用Kromasil C18柱(4.6mm×250mm,5μm);流动相:乙腈(A)-水(B),梯度洗脱(0～35min,45%A→70%A;35～40min,70%A→90%A;40～60min,90%A→95%A),流速1.0mL·min-1;检测波长214nm;柱温25℃。结果:莪术二酮、莪术醇、吉玛酮、呋喃二烯、β-榄香烯分别在27.88～278.8,20.42～204.2,3.475～34.75,5.380～53.80,10.12～101.2μg·mL-1范围内线性关系良好,r≥0.999 6。平均加样回收率(RSD)分别为97.36%(1.85%),97.96%(2.30%),97.47%(2.02%),98.10%(2.38%),98.94%(2.01%)。结论:本方法操作简便、准确可靠,适用于莪术中5种倍半萜类成分的定量分析。
OBJECTIVE To establish an HPLC method for the simultaneous determination of five sesquiterpene content of curdi- one, curcumol, gerrnacrone, furanodiene and β-elemene in Curcumae Rhizoma from different areas. METHODS The separation was performed on a Kromasil C18 column （4. 6 mm × 250 mm, 5 μm） with the mobile phase consisting of acetonitrile （A）-water （B） by gradient elutiorL The gradient condition was as follows： 0 - 35 min, 45% A→70% A;35 - 40 min,70% A→90% A;40 - 60 min, 90% A→95% A. The flow rate was 1.0 mL· min^- 1. The column temperature was set at 25℃ and the UV detection wavelength was 214 nm. RNSULTS The linear ranges and correlation coefficients of eurdione, curcumol, germacrone, furanodiene and β-elemene were 27. 88 - 278. 8, 20. 42 - 204. 2, 3. 475 - 34. 75, 5. 380 - 53. 80, 10. 12 - 101.2μg. mL ^-1 and r≥0. 999 6. The average recoveries were 97. 36%, 97. 96%, 97. 47% ,98. 70% and 99. 05% with corresponding RSD of 1.85%0, 2. 30%, 2. 02%, 2. 40% and 1.91%. CONCLUSION The method is simple, accurate, reproducibility and can be used to determine curdione, curcumol, germacrone, furan odiene and β-elemene in Curcumae Rhizoma from different areas.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2012年第24期1954-1957,共4页
Chinese Journal of Hospital Pharmacy
基金
江苏省教育厅重大项目
中药醋制入肝效应及炮制机理研究(编号:09KJA360001)
中国中医科学院中药研究所基本科研业务费资助课题(编号:2011ZDXK-02)
