摘要
目的研究大黄炭定性、定量检测方法,建立大黄炒炭饮片的质量标准。方法采用HPLC法测定大黄炭中大黄酚、大黄素含量。色谱柱填料为Kromasil-C18(4.6mm×150mm,5μm),检测波长254nm。流动相为甲醇-0.1%磷酸溶液(85∶15),流速1.0mL/min。结果平均回收率分别为大黄素98.43%、大黄酚98.32%,RSD分别为1.17%、1.02%。结论本方法重现性好、稳定、简单,可用于大黄炮制工艺及质量控制。
Objective To establish the quality standard of charred Radix et Rhizoma Rhei. Kromasfl-C18 column （4.6 mm× 150 mm, 5 μm） was used with mobile phase of methanol-0. 1% phosphate acid （85 ; 15）, 1.0 mL/min, and detection at 254 nm. Result Average recoveries of charred Radix et Rhizoma Rhei： Emodin was 98.43% and Chrysophanol was 98.32%, RSD was 1.17% and 1.02% respectively. Conclusion This method is simple. The result is accurate and reproducible. It can be used in the study of the processing technics of Radix et Rhizoma Rhei and the quality control of charred Radix et Rhizoma Rhei.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第6期50-53,共4页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家科技部"九五"攻关项目(99-929-01-41)
关键词
大黄炭
含量测定
质量标准
charred Radix et Rhizoma Rhei
determination
quality standard
