摘要
目的分析不同产地莪术药材中莪术烯醇、莪术酮和吉马酮含量的差异。方法采用ZORBAX RX-C18柱(4.6mm×250mm,5μm),柱温:35℃,流动相:甲醇-水(含0.4%的醋酸)-异丙醇,采用梯度洗脱程序,检测波长:254nm,流速:1mL·min-1。结果莪术烯醇、莪术酮和吉马酮质量浓度分别在5.18～155.5,5.902～177.1和6.19～185.7mg·L-1内线性关系良好,平均回收率分别为99.6%,101.9%和99.8%,其中四川仙森公司蓬莪术中的莪术烯醇、莪术酮和吉马酮RSD分别为1.31%,0.94%和2.18%(n=3)。结论本法简便、准确、重复性好,可用于控制莪术药材质量。
OBJECTIVE To determine three effective constituents of curcumenol,curzerenone and germacrone in Rhizoma Curcumae from different areas. METHODS A HPLC method was established using a ODS-C18 column （4.6 mm×250 mm,5 μm）.The mobile phase by a gradient program was methanol-water （0.4% acetic acid）-isopropanol. The flow rate was 1.0 mL·min-1 with UV detection at 254 nm. The column temperature was 35 ℃. RESULTS The method displayed good linearity of curcumenol,curzerenone and germacrone within the concentration ranges of 5.18-155.5,5.902-177.1 and 6.19-185.7 mg·L-1. The average recovery was 99.6%,101.9% and 99.8% for curcumenol,curzerenone and germacrone respectively. The RSDs the three constituents from Curcuma phaeocaulis Valeton in Sichuan Xiansen company was 1.31%,0.94% and 2.18% respectively （n=3）. CONCLUSION This method is convenient,accurate and can be used to identify and evaluate the quality of Rhizoma Curcumae.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2009年第22期1742-1744,共3页
Chinese Pharmaceutical Journal
