摘要
目的：建立HPLC法测定莪术油及其注射液中6种成分含量。方法：采用Waters Symmetry C18色谱柱（4．6mm×250mm，5um），流动相甲醇-水，梯度洗脱；柱温30℃，检测波长215nm。结果：6种成分在该色谱条件下分离良好。莪术二酮、莪术醇、栊牛儿酮、莪术烯、呋喃二烯和β-榄香烯分别在1．74～347．00，2．38～475．00，2．49～497．00，2．07～826．00，5．05～1009．00，4．78～955．00mg·L^-1线性关系良好（r≥0．9996），平均回收率均大于95％（RSD〈3．0％，n=9）。结论：该方法准确、可靠、重复性良好，可用于莪术油及其注射液的质量控制。
Objective： To establish an HPLC method to simultaneously determine six ingredients in zedoary turmeric oil and its related injections. Method： HPLC analysis was performed on Waters Symmetry C18 （4. 6 mm × 250 mm,5 um）. The mobile phase was composed of methanol and water solution in gradient elution mode with at a flow rate of 1.0 mL · min^-1. The column temperature was 30 ℃ and the UV detection wavelength was 215 nm. Result： The six ingredients were separated well. The linear ranges of curdione, curcumol, germacrone, curzerene, furanodiene and β-elemene were 1.74-347.00, 2.38-475.00, 2. 49-497.00, 2.07-826.00, 5. 05-1 009.00,4. 78-955. 00 mg · L^-1 （r≥0. 999 6） ,respectively. The average recoveries were above 95% （ RSD 〈 3.0% ,n =9）. Conclusion： The method is accurate, reliable and reproducible,it can be used for the quality control of zedoary turmeric oil and its related injections.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第5期593-597,共5页
China Journal of Chinese Materia Medica
基金
国家"十一五"科技支撑计划项目(2006BAI14B01)
关键词
HPLC
莪术油
莪术油注射液
含量测定
HPLC
zedoary turmeric oil
zedoary turmeric oil injection
quantitative determination
