摘要
目的:建立以气相色谱法测定不同产地醋莪术饮片中β-榄香烯含量的方法。方法:以水杨酸甲酯为内标物,采用HP-5弹性石英毛细管色谱柱,程序升温,氢火焰离子化检测器,气化室温度为240℃,检测室温度为250℃,不分流进样,进样量为1μL。结果:β-榄香烯进样量在0.01507～0.15070μg范围内同其与水杨酸甲酯峰面积的比值呈良好线性关系(r=0.9996);平均回收率为96.13%,RSD=1.37%(n=9)。温郁金中的β-榄香烯含量相对较高。结论:本方法可作为不同产地醋莪术饮片中β-榄香烯的含量测定方法。
OBJECTIVE：To establish GC method for the content determination of β-elemene in Rhizoma Curcumae decoction pieces from different habitats.METHODS：The determination condition were as follows：HP-5 capillary column（30 m×0.53 mm×1.0 μm）,methyl salicylate as the internal standard,itemperature programmng,FID,splitless and injection volume of 1 μL.The temperature of gasification room was set at 240 ℃;the temperature of detecting room was set at 250 ℃.RESULTS：The linear range for β-elemene was 0.015 07～0.150 70 μg（r=0.999 6） with an average recovery of 96.13%（RSD=1.37%,n=9）.β-elemene took up relative high proprotion in Curcuma wenyujin.CONCLUSION：The method can be used for the content determination of β-elemene Rhizoma Curcumae in decoction pieces.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第39期3697-3699,共3页
China Pharmacy
基金
<中国药典>2010版(一部)标准研究课题(YZ-107)
关键词
醋莪术饮片
Β-榄香烯
气相色谱法
含量测定
Rhizoma Curcumae decoction pieces
β-elemene
GC
Content determination
