摘要
目的 探讨运用白细胞粘附分子LFA-1单克隆抗体防治烧伤休克及改善微循环的作用机制。方法 复制大鼠烧伤休克模型，测量微静脉血液流速、口径和流量，镜下观察微静脉白细胞粘附数并记录动物存活时间。结果 单抗能减缓烧伤休克大鼠平均动脉压和微静脉血流速度的下降趋势，显著降低微静脉的白细胞附壁粘着数，明显延长动物的存活时间。结论 运用LFA-1单抗能阻断白细胞与内皮细胞的粘附、减少白细胞嵌塞微静脉以改善烧伤休克微循环和保护组织细胞。
Objective To detect the effect of LFA-1 monoclonal antibody (mAb) on microcirculation disorder in burn shock rats. Methods The flow velocity and diameter of the venule were measured by RBC tracking corrclator and IV550 model video microscaler from burn shock models of rats. The number of leukocytes adhering on the venule wall was calculated under microscope. The animals' survival time was observed. Result LFA-1 mAb could attenuate the falling of mean arterial pressure, significantly reduce the number of leukocytes adhering on the venule wall, and obviously prolong the animals, survival time. Conclusion LFA-1 mAb can decrease the number of leukocytes adhering to endothelial cells, attenuate the tether of leukocytes to venule and improve microcirculation in burn chock of rats.
出处
《第一军医大学学报》
CSCD
2000年第3期214-215,225,共3页
Journal of First Military Medical University
基金
广东省自然科学基金资助(940303)
