摘要
目的探讨虎杖甙改善微循环的作用机理。方法在细胞液流室中，观察虎杖甙（ＰＤ）对血液中性粒细胞与培养的血管内皮细胞粘附性的影响。结果在正常情况下，随着切应力增大，白细胞与内皮细胞粘附率逐渐下降，但ＩＬ－８处理组和失血性休克组的白细胞，其与内皮的粘附率都明显高于对照组。而这两组的白细胞如果事先与ＰＤ温育３０ｍｉｎ后，再与内皮细胞作用，则白细胞与内皮的粘附率明显下降到接近正常对照水平。如果中性粒细胞与内皮细胞先粘附并以一定切应力灌流后再加入ＰＤ溶液孵育，也发现ＰＤ能显著降低白细胞与内皮的粘附率。结论ＰＤ能通过直接降低白细胞对血管内皮的粘附性而改善微循环。
Objective To investigate the mechanism of Polydatin (PD) on improving the microcirculation. Methods By using cell flow chamber technique and video recording system,we observed the changes of adhesion rate between neutrophils (WBC) and endothelial cells (EC) treated by PD at the shear stress from 0.311 to 5.621 dyn/cm^2. Results It was found that the adhesion rate of WBC-EC decreased with the increase of shear stress. But the adhesion rate in IL-8 treated group and shock group was higher than that of control group at the same shear stress. When the neutrophils from IL-8 group and shock group were incubated with PD,the adhesion rate of WBC-EC was recovered to the normal control level.And when PD was administered after WBC-EC incubation and perfusion,the adhesion rate was also reduced. Conclusion PD could reduce the adhesion of neutrophil-EC directly,which may be one of the reasons that PD can improve the microcirculation.
出处
《中国微循环》
1999年第2期82-84,共3页
Journal of Chinese Microcirculation
基金
国家自然科学基金!39870936
关键词
虎杖甙
细胞粘附
内皮细胞
Polydatin Cell adhesion Endothelial cells Shock
