摘要
[目的]研究花生活性肽的分子量分布及其与抗氧化活性的关系。[方法]采用截留分子量8.0、3.5、1.0 kD的透析袋对花生蛋白酶水解液进行分级分离,得到不同分子量范围的抗氧化肽,分别测定其清除自由基的能力,并与Tricine-SDS-PAGE技术相结合,考察花生蛋白酶解物的分子量分布。[结果]分子量范围在1.0 kD以下的多肽混合物含量分别为碱性蛋白酶和风味酶的总水解物的76.21%和83.42%,不同分子量范围的水解物均具有一定的清除自由基的能力,且随分子量的减小而增大,分子量范围在1.0 kD以下的组分对碱性蛋白酶和风味酶中的自由基的清除能力最强,分别为(87.41±0.66)%和(67.88±0.48)%。[结论]花生活性肽的抗氧化能力较强,尤其是分子量1.0 kD以下的短肽,具有较好的开发应用前景。
[Objective] To prepare peanut bioactive peptides and analyze their molecular weight composition and antioxidant activity.[Method]The dialysis bag of 8.0,3.5 and 1.0 kD were used to classify the hydrolyzate derived from alcalase and flavourzyme,peanut bioactive peptides of different molecular weight were obtained and then their scavenging capacity of free radicals was measured.The molecular weight composition was studied by Tricine-SDS-PAGE.[Result] The content of peptides1.0 kD were 76.21% and 83.42% in the total hydrolyzate from alcalase and flavourzyme respectively.All hydrolyzate with different molecular grades showed free radical scavenging capacity,which was increased with the reduction of molecular mass.The peptides1.0 kD exhibited higher radical scavenging capacity of（87.41±0.66）%（alcalase） and（67.88±0.48）%（flavourzyme）,respectively.[Conclusion] Peanut bioactive peptides had strong effect of antioxidation capacity,especially that1.0 kD,which had great prospect.
出处
《安徽农业科学》
CAS
2012年第21期11041-11043,共3页
Journal of Anhui Agricultural Sciences
基金
国家科技部2011年度农业科技成果转化资金基金项目(2011C 3002001)
