摘要
目的:观察花生肽对小鼠运动能力和心肌抗氧化能力的影响。方法:昆明种小鼠随机分为6组,安静对照组、安静花生肽样品(400 mg·kg-1)组、运动对照组、运动花生肽样品高、中、低三个剂量组。运动各组进行连续4周的游泳训练,连续给药至第28天进行一次负重耐力实验。测定心肌丙二醛(MDA)含量和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性,测定血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、肌酸激酶(CK)活性。结果:花生肽处理组心肌MDA含量明显降低,心肌SOD、GSH-Px和CAT活性均高于相应的对照组。花生肽处理组小鼠运动能力显著增强。运动花生肽中剂量、运动花生肽高剂量组和运动对照组相比,血清中GPT、GOT和CK活性显著降低。结论:花生肽通过增强心肌抗氧化能力,减轻了剧烈运动对心脏造成的损伤,来发挥它的心脏保护作用。
Objective ： To study the effects of peanut peptide on the sport activity and antioxidant capacity of mouse. Method： Sixty Kunming mice were randomly divided into six group, which were named normal group, peanut peptide group, exercised control group, exercised ＋ peanut peptide in low content, exercised ＋ peanut peptide in middle content, and exercised ＋ peanut peptide in high content. The mouses of exercised groups were obtained swimming training for 4 weeks. At the last day of the experiment, the mice were experienced exhausted swimming bearing load. And then the content of MDA and the activity of SOD, CAT, GSH - Px in the heart tissue were detected with the correponding kit. The enzyme activity of GPT, GOT and CK in serum were assayed with kit, too. Results：The sports activity of mice treated with peanut pepfide increased obviously compared with the control. The level of MDA decreased and the activity of antioxidant enzymes （SOD, CAT, GSH-Px ） in heart tissue elevated in peanut peptide groups, In the same time, the activity of cardiac marker enzyme in serum decreased obviously in exercised ＋ peanut peptide groups of middle and high content. Conclusion ： Peanut peptide might alleviate the cardiac damage induced by strenuous exercise and protect heart through enhancing myocardial antioxidant capacity.
出处
《山东体育科技》
北大核心
2013年第5期75-77,共3页
Shandong Sports Science & Technology
关键词
花生肽
小鼠
心脏保护
抗氧化
peanut peptide
mouse
heart protection
antioxidant capacity
