摘要
采用响应面分析法,优化胃蛋白酶酶解王浆蛋白制备抗氧化肽的工艺条件,以1,1-二苯基-2-苦基苯肼(DPPH)清除率为响应值,研究了酶与底物的比值([E]/[S])、底物浓度以及酶解时间对蜂王浆酶解物抗氧化的影响。结果表明,最终确定出制备抗氧化肽的最优酶解条件为,[E]/[S]2.24%、底物浓度1.61%、酶解时间3.7 h,在该条件下制备的王浆蛋白酶解物的DPPH自由基清除率为47.84%。
In order to optimize enzymolysis technology of antioxidant peptides from royal jelly protein with pepsin,the radical scavenging activity of DPPH were taken as response values to analyze the effect of ratio of enzyme to substrate（）,substrate concentration and hydrolysis time on the technology of antioxidant peptides from royal jelly.The result showed that the optimum conditions to achieve the maximum antioxidant ability were as follows： 2.24 %,substrate concentration 1.61 %,enzymolysis time 3.7 h.Under such conditions,DPPH radical scavenging activity of antioxidant peptides reached 47.84 %.
出处
《西南农业学报》
CSCD
北大核心
2012年第5期1652-1656,共5页
Southwest China Journal of Agricultural Sciences
基金
现代农业产业技术体系建设专项资金资助(CARS-45-KXJ19)
关键词
王浆蛋白
抗氧化肽
响应面分析法
Royal jelly protein
Antioxidant peptides
Response surface method
