摘要
目的:建立HPLC法同时测定消炎片中绿原酸、秦皮乙素和咖啡酸的含量。方法:采用Agilent Eclipse XDB-C_(18)(250mm×4.6 mm,5μm)色谱柱,以甲醇-乙腈-0.04%磷酸溶液为流动相,梯度洗脱;检测波长为328 nm;流速1.0 ml·min^(-1);进样量20μl;柱温35℃。结果:绿原酸、秦皮乙素和咖啡酸的线性范围分别为1.616～40.400μg·ml^(-1)(r=0.999 9,n=7)、5.164～129.100μg·ml^(-1)(r=1.000 0,n=7)和1.344～26.880μg·ml^(-1)(r=1.000 0,n=7);提取回收率分别为99.08%(RSD=0.6%,n=6)、98.81%(RSD=0.4%,n=6)和98.92%(RSD=0.9%,n=6)。结论:本法操作简便、快速、结果准确。
Objective： To establish a RP - HPLC method for the simultaneous determination of chlorogenic acid,esculetin and caffeic acid in Xiaoyan tablets. Method： An Agilent Eclipse XDBTC18 （250mm×4.6mm,5μm） column was used. The mobile phase was methanol - acetonitrile-0. 04% H3 PO4 with gradient elution and the detection wavelength was 328 nm, the flow rate was 1.0 ml. min-1, the injection volume was 20 μl and the column temperature was 35℃. Result： The linear response of chlorogenic acid range was from 1.616-40. 400μg·ml-1 （ r = 0. 999 9, n = 7 ）, and that for esculetin and caffeic acid was from 5. 164-129. 100 Ixg＂ ml - 1 （ r = 1. 000 0, n =7） and 1. 344-26. 880μg·ml-1 （ r = 1. 000 0, n = 7 ）, respectively. The average recovery was 99. 08% （ RSD = 0.6% , n = 6）, 98. 81% （ RSD = 0.4%, n = 6 ） and 98.92% （ RSD = 0. 9%, n = 6 ） accordingly. Conclusion： The method is convenient, quick and accurate.
出处
《中国药师》
CAS
2011年第12期1757-1758,共2页
China Pharmacist
