摘要
采用高效液相色谱法测定珍菊降压片中野菊花绿原酸的含量。选用Kromosil^(TM)十八烷基硅烷键合硅胶色谱柱(150mm×4.6mm.id.,5μm),乙腈-0.4%磷酸溶液(13∶87)为流动相,流速为1.0mL/min,检测波长为327nm。线性范围为0.8～6.4μg/mL(r=0.9992),回收率为103.4%,RSD为2.4%(n=5)。
The method for determining the content of chloralgenic acid in Zhenjujiangya tablets by HPLC was established. Chromatographic colum was Kromosil^TM ODS -2 column（ 150 mm ×4.6 mmi. d. ,5 μm） , mobile phase was acetonitrile -0.4% phosphonic acid（13：87） with flow rate of 1.0 mL/min, detection wave length was 327 nm. There was good linearity in the range of 0. 8 - 6.4 μg/mL（r =0.9992）, the average recovery of chloralgenic acid was 103.4% and the RSD was 2.4% （n =5）.
出处
《化学分析计量》
CAS
2007年第1期48-50,共3页
Chemical Analysis And Meterage
