摘要
目的:用HPLC法测定灯盏细辛中绿原酸和咖啡酸的含量。方法:色谱柱为C_(18)柱(200 mm×4.6 mm,5μm);流动相为甲醇-1.25%冰醋酸溶液(15:85),检测波长326 nm。结果:绿原酸在0.04～8.15μg、咖啡酸在0.01～2.55μg范围内线性关系良好,平均回收率分别为99.23%,96.92%(RSD=1.56%,RSD=1.49%,n=6)。结论:为采收加工灯盏细辛和进一步研究其质量控制方法提供参考。
Objective： To establish a method for the determination of ehlorogenie acid and eaffeie acid in Erigerion breviscapus. Method： A C18 column （200 mm ×4.6 mm,5 μm） was used with a mobice phase of methanol-1.25% glacial-acetic acid （ 15：85 ） and UV detection wavelengeth was at 326 nm. Result： There was a good linear range of 0.04 - 8.15 μg for chlorogenic acid and a range of 0.01 -2.55 μg for caffeic acid; the average recovery for chlorogenic acid was 99.23% （RSD = 1.56% , n = 6 ）, for eaffeic acid was 96.92% （ RSD = 1.49% ,n = 6）. Conclusion： This method can be applied to the determination of chlorogenic acid acid caffeic acid in Erigerion breviscapus.
出处
《中国药师》
CAS
2010年第5期701-702,共2页
China Pharmacist
关键词
灯盏细辛
绿原酸
咖啡酸
HPLC
Erigerion breviscapus
Chlorogenic acid
Caffeic acid
HPLC
