摘要
为阐明低氧性肺动脉高压大鼠肺组织 型胶原的变化及其与肺动脉压的关系 ,分别用右心导管法检测肺动脉压力和肺循环阻力等血流动力学指标 ,以链亲合素过氧化物酶法观察低氧大鼠肺动脉、支气管和肺间质 型胶原的分布变化 ,用图像分析技术检测肺小动脉的形态改变和胶原组化染色灰度扫描。结果 :低氧大鼠肺动脉压明显升高 ,肺小动脉管壁增厚 ,管腔狭窄 ,反映管壁增厚的两个指标管壁厚度占血管外径的百分比 (35 .83±3.5 5 )和管壁面积占血管总面积的百分比 (5 9.6 8± 4.90 )均显著高于对照组的 1 6 .35± 2 .6 4和 2 6 .83± 3.40 (P<0 .0 1 )。 型胶原表达明显增强 ,主要分布在肺动脉的外膜 ,经灰度扫描肺动脉壁的阳性程度 (2 .5 5± 0 .34 )明显高于对照组 (1 .2 4± 0 .2 1 ,P<0 .0 1 ) ,且与管壁厚度占血管外径的百分比 (35 .83± 3.5 5 )及平均肺动脉压 (3.95±0 .43k Pa)呈直线正相关 (r=0 .85 ,P<0 .0 1 ;r=0 .6 3,P<0 .0 5 ) ,说明肺动脉
The changes of type Ⅰ collagen in the lung tissues of rats with hypoxic pulmonary hypertension (HPH) and its relationship to the mean pulmonary arterial pressure (mPAP) were investigated. The blood dynamic indexes of pulmonary circulation were measured by using Swan Ganz. The distribution of type Ⅰ collagen in pulmonary arteries, bronchi and plumonary interstitial was observed by using streptavidin peroxidase method (SP). The morphologic changes of small pulmonary arteries and the level of type Ⅰ collagen were determined by image pattern analysis technique and gray scale scanning, respectively. The results showed, compared with the controls, mPAP of the hypoxic rats elevated (from 2.12±0.25 to 3.95±0.43 kPa, P <0.01), their small pulmonary arteries, walls thickened (MT% from 16.35±2.64 to 35.83±3 55, MA% from 26.83±3.40 to 59 68±4.90, P <0.01) and lumen narrowed. The distribution of typeⅠ collagen was mainly in the external layer of pulmonary arterial walls; the positive degrees of gray scale scanning increased and had a positive linear relationship to the mPAP and MT%. These findings suggest that the increase of typeⅠ collagen contents in pulmonary arteries is closely related to the development of HPH.
出处
《华西医科大学学报》
CSCD
2000年第1期34-36,61,共4页
Journal of West China University of Medical Sciences
