摘要
目的了解低氧肺动脉高压时肺内表皮生长因子受体(EGFR)与肺血管重建的关系。方法将20只雄性Wistar大鼠分为低氧性肺动脉高压组(低氧组)(n=10)和正常对照组(n=10),肺动脉高压组以常压低氧复制大鼠肺动脉高压模型,以微导管法测定各组大鼠肺动脉压,采用免疫组织化学和原位杂交法检测各组大鼠肺内EGFR蛋白和EGFR mRNA的表达,对肺组织切片进行图像分析。结果低氧3周后,低氧组大鼠形成明显的肺动脉高压,管壁增厚,管腔狭窄,低氧组大鼠肺动脉压(2.86±0.39)kPa、右心室肥厚指数﹝RV/(LV+S)﹞为(43.53±3.38)%、管壁厚度占外径的百分比(WT%)为(35.24±11.2)、管壁面积占血管总面积的百分比(WA%)为(55.09±12.38),分别与正常对照组(1.35±0.28)kPa、(23.68±3.48)%、(23.63±9.74)%和(41.62±12.83)%相比明显升高(P<0.01);肺小动脉EGFR蛋白免疫阳性染色在低氧组大鼠为(1.48±0.07),与正常对照组(1.09±0.02)相比明显增强(P<0.01),低氧组大鼠EGFR mRNA阳性染色为(1.43±0.05),与正常对照组(1.10±0.04)相比明显增强(P<0.01)。结论低氧所致EGFR的合成增多在低氧性肺血管重建和肺动脉高压的发病过程中起一定的作用。
【Objective】 To test the role of epidermal growth factor receptor（EGFR） in the development of hypoxic pulmonary hypertension.【Methods】 Twenty male Wistar rats were divided into two groups and exposed to air and isobaric hypoxia for 3 weeks,respectively.The pulmonary artery pressure was measured by right cardiac catheterization.The expression of EGFR protein and EGFR mRNA in the lungs of rats were measured by immunohistochemical staining and in situ hybridization.The histological sections of the lungs were examined using a computerized image analyzer.【Results】 The pulmonary artery pressure in the exposed rats increased.The chronic hypoxia also elicited the thicking of the wall and the narrowing of the lumen of pulmonary arterioles.It led to the increases of the pulmonary artery pressure,the index of right ventricular hypertrophy（RV/（LV＋S）,the ratio of vascular wall thickness/vascular external diameter（WA%） and the ratio of vascular wall area/total vascular area（WT%） compared to the controls PAP（2.86±0.39） kPa vs（1.35±0.28 kPa）;（RV/（LV＋S）：（43.53±3.38） vs（23.68±3.48）;WT%：（35.24±11.2） vs（23.63±9.74）;WA%：（55.09±12.38） vs（41.62±12.83） respectively,P 0.05）.The positive staining of EGFR protein and EGFRmRNA in the wall of pulmonary arteriole of the exposed rats were significantly stronger than those of controls（P 0.01）.【Conclusions】 The EGFR may play an important role in the pathogenesis process of hypoxic pulmonary vascular remodeling and pulmonary hypertension.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2012年第23期8-12,共5页
China Journal of Modern Medicine
基金
国家自然科学基金(No:30960142)
关键词
低氧
肺动脉高压
肺血管重建
表皮生长因子受体
hypoxia
pulmonary hypertension
pulmonary vascular remolding
epidermal growth factor receptor（EGFR）
