摘要
目的了解低氧肺动脉高压时肺内结缔组织生长因子(CTGF)与肺血管重建的关系。方法将20只雄性Wistar大鼠分为低氧性肺动脉高压组(n=10)和对照组(n=10)两组,肺动脉高压组以常压低氧建立大鼠肺动脉高压模型,以微导管法测定各组大鼠肺动脉压,采用免疫组化法检测各组大鼠肺内CTGF和Ⅰ型胶原的表达,对肺组织切片进行图象分析。结果低氧3周后,低氧组大鼠形成明显的肺动脉高压,管壁增厚和管腔狭窄,低氧组大鼠肺动脉压(2.89±0.41)kP,右心室肥厚指数[RV/(LV+S)]为41.01±2.79、管壁厚度占外径的百分比(WT%)为35.13±10.31,管壁面积占血管总面积的百分比(WA%)为54.18±11.46,分别与正常对照组(1.37±0.26)kP、24.59±3.51、23.66±9.74和40.59±13.87相比明显升高(P<0.01);肺小动脉CTGF免疫阳性染色在低氧组大鼠为1.32±0.05,与正常对照组1.10±0.04相比明显增强(P<0.01),低氧组大鼠Ⅰ型胶原免疫阳性染色为1.30±0.04,与正常对照组1.14±0.05相比明显增强(P<0.01)。结论低氧所致CTGF的合成增多在低氧性肺血管重建和肺动脉高压的发病过程中起一定的作用。
Objective： To test the role of connective tissue growth factor （CTGF） in the development of hypoxic pulmonary hypertension. Methods： Twenty male Wistar rats were divided into two groups and exposed to air and isobaric hypoxia for 3 weeks, respectively. The pulmonary artery pressure was measured by right cardiac catheterization. The expression of CTGF and collagen Ⅰ in the lungs of rats were measured by immunohistochemical staining.The histological sections of the lungs were examined using a computerized image analyzer. Results： The pulmonary artery pressure in the exposed rats increased. The chronic hypoxia also elicited the thicking of the wall and the narrowing of the lumen of pulmonary arterioles. It led to the increases of the pulmonary artery pressure , the index of right ventficular hypertrophy [ RV/ （LV ＋ S）], the ratio of vascular wall thickness/vascular external diameter （WA%） and the ratio of vascular wall area/total vascular area （WT%） compared to the controls （PAP2.89 ± 0.41kP vs 1.37±0.26kP; RV/ （LV＋S）： 41.01±2.79vs24.59±3.51; WT%： 35.13±10.31vs23.66±9.74; WA%.： 54.18 ± 11.46 vs40.59± 13.87 respectively, p〈 0.05） .The positive staining of CTGF and collagen Ⅰ in the wall of pulmonary arteriole of the exposed rats were significandy stronger than those of controls （ P 〈 0.01） .Conclusions： The CTGF may play an important role in the pathogenesis process of hypoxie pulmonary vascular remodeling and pulmonary hypertension.
出处
《华西医学》
CAS
2007年第2期318-320,共3页
West China Medical Journal
关键词
低氧
肺动脉高压
肺血管重建
结缔组织生长因子
Hypoxia
Puhnonary hypertension
Pulmonary vascular remolding
Connective tissue growth factor
