摘要
制备了基于多壁碳纳米管-硫堇/Nafion纳米复合物的人IgG免疫传感器。阻抗谱、循环伏安研究表明,该免疫传感器对人IgG的检测具有优异的性能,其对人免疫球蛋白G(IgG)浓度的定量测定线性范围为1.0～200μg/L,检出限为0.25μg/L,线性相关系数R=0.9950。该免疫传感器可用于对人血清中IgG的检测。
An immunosensor for human IgG based on multi-wall carbon nanotube-thionine/Nafion nanocomposite film modified electrodes was prepared. On the basis of the results of electrochemical impedance spectroscopy （EIS） and cyclic voltammetry （CV）, it was found that the as-propared electrochemical immunosensor presented excellent performances to the detection of human IgG. The linear range of the immunosensor was 1.0 - 200 μg/ L with a correlation coefficient of 0. 9950, and the detection limit was 0. 25 μg/L. The results suggest that the aspropared electrochemical immunosensor will be promising in the applications of point-of-care diagnostics and clinical screening of cancer biomarkers.
出处
《分析试验室》
CAS
CSCD
北大核心
2011年第11期81-84,共4页
Chinese Journal of Analysis Laboratory
基金
重庆市教委科学技术研究资助项目(No.KJ101315)资助
关键词
碳纳米管
硫堇
免疫传感器
Carbon nanotubes
Thionine
Immunosensor
