摘要
利用甲醛脱氢酶和羧基化多壁碳纳米管修饰的丝网印刷电极,制备了基于还原型辅酶Ⅰ检测的甲醛生物传感器,并优化了传感器的检测条件。结果表明,此传感器对甲醛有较好的电催化氧化作用,显著降低了甲醛的氧化峰电位。在0.001～11mmol/L范围内,响应电流与甲醛的浓度线性相关,其线性回归方程为i(μA)=0.944c(mmol/L)+0.0623,相关系数为0.9934,响应时间约为20 s,检出限为0.2μmol/L(S/N=3)。
A novel formaldehyde biosensor based on the detection of reduced coenzymeⅠby using the formaldehyde dehydrogenase and carboxyl multi-walled carbon nanotubes modified screen printed electrode was reported.The best detection conditions in experiments were examined.The experiment results showed that the sensor had good electrocatalytic oxidization function to formaldehyde,and it significantly reduced the formaldehyde oxidation peak voltage.The concentrate of formaldehyde in the range of 1 μmol / L to 11 mmol / L presented excellent linear relationship to its peak currents and its the linear regression equation was i（μA） = 0.944c（mmol/L） ＋0.0623,with a correlation coefficient of 0.9934.The response time was about 20 s.The detection limit was 0.2 μmol / L（S / N = 3）.
出处
《分析化学》
SCIE
CAS
CSCD
北大核心
2012年第6期909-914,共6页
Chinese Journal of Analytical Chemistry
关键词
碳纳米管
甲醛脱氢酶
还原型辅酶Ⅰ
丝网印刷电极
Carbon nanotubes； Formaldehyde dehydrogenase； Reduced coenzymeⅠ； Screen-printed electrodes
