摘要
研究了依思明蓝和牛血清白蛋白结合的共振散射光谱特征.实验结果表明,在pH=3.78水溶液中,血清白蛋白可与染料探针依思明蓝通过疏水作用结合并产生以367.4-nm为特征峰的共振光散射(RLS)增强光谱.在该特征波长下测定的增强共振光散射强度(ΔIRLS)与血清白蛋白浓度在一定范围内具有线性关系.据此建立了痕量血清蛋白的共振光散射分析法.当依思明蓝的浓度为1.5×10-5mol/L时,牛血清白蛋白,人血清白蛋白以及IgG的检测限分别可达2.34-ng/mL,6.86-ng/mL和10.9-ng/mL.基于共振光散射数据及Scatchard图,获得依思明蓝与牛血清白蛋白和人血清白蛋白的结合常数(K)以及结合位点数(n)分别为2.90×105L/mol,2.9和2.96×105L/mol,0.35.
Studies on binding of Isamin Blue （IB） and BSA are presented here. At pH=3.78, the static interaction of IB with BSA occurs and results in greatly enhanced resonance light scattering（RLS） signals characterized by a peak at 367.4 nm. It was found that the enhanced RLS intensity （△IRLS） at 367.4 nm is proportional to the concentration of BSA, and thus a new method for trace BSA analysis could be estab lished accordingly. The limit of determination （3σ） is 2.34 ng/mL when 1.5×10^-5 mol/L IB was em ployedfor BSA, 6.86 ng/mL for HSA and 10.9 ng/mL for IgG, respectively. Based on the measurements of the RLS data, a Scatchard plot concerning the interaction between BSA-IB and HSA-IB could be constructed and the binding number （n） and binding constant （K） are 2.9, 2.90×10^5 L/mol and 0.35, 2.96× 10^5 L/mol, respectively.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第3期70-75,共6页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家杰出青年基金资助项目(20425517)
教育部春晖计划资助项目(渝教外:[2004]724)
关键词
依思明蓝
牛血清白蛋白
人血清白蛋白
共振光散射
Isamin Blue （IB）
bovine serum protein（BSA）
human serum protein（HSA）
Resonance light scattering （RLS）
