摘要
随着生物及药物分析领域的不断扩展,发展高灵敏度及高选择性的分析手段以解决复杂样品体系中低浓度待测物的分析测试问题是十分迫切的需求.电化学发光分析方法由于具有线性范围宽,灵敏度高及可控性强等优点,是处理低浓度样品的有效工具.这种方法与免疫传感技术相结合,有利于实现生物体液等复杂样品中极低含量生化物质与药物的高选择性、高灵敏度检测.本文综述了电化学发光免疫传感技术的发展状况,介绍了近年来在电化学发光免疫传感中出现的新型固相载体、电化学发光探针和共反应物、以及多组分免疫传感技术等,并对其在生物药物分析中的应用情况进行了总结.
With the development of biological and pharmaceutical analysis, assay technique with high sensitivity and selectivity, and suitable for multianalyte assay, is required for complicated sample matrix. Due to its advantages such as wide linear range, high sensitivity and good controllability, electrochemiluminescence （ECL） analysis technique becomes a useful tool for low-level analyte. Combined with immunosensing, it shows great promise in assaying low-level biological substances and drugs in complicated matrix such as biological fluid. This review presents the development of ECL immunosensing technique in recent years. Some novel substrates, ECL probes, co-reactants, multianalyte immunosensing methods and their application in biological and pharmaceutical analysis have been introduced.
出处
《中国科学：化学》
CAS
CSCD
北大核心
2011年第5期773-784,共12页
SCIENTIA SINICA Chimica
基金
国家自然科学基金(20805036
21035005)
高等学校博士学科点专项科研基金(200806351006)
重庆市自然科学基金(CSTC
2009BB5116)资助
关键词
电化学发光
免疫传感器
电化学发光探针
共反应物
生物药物分析
electrochemiluminescence
immunosensor
electrochemiluminescent probe
co-reactant
biological and pharmaceutical analysis
