摘要
利用壳聚糖与纳米金良好的生物相容性及蛋白固定能力,制备了兼具导电性和透光性的人免疫球蛋白G(IgG)修饰膜,用于修饰玻碳电极,研制了新型电化学发光免疫传感器,并通过扫描电镜(SEM)及交流阻抗技术(EIS)考查了传感器表面性质.基于竞争免疫分析模式,以Ru(bpy)32+标记的羊抗人IgG为发光示踪物,采用新型共反应剂二丁基乙醇胺(DBAE)对光信号进行放大,建立了人IgG的检测方法,线性范围20ngmL-1～1.0μgmL-1,检测限6.5ngmL-1.将该电化学发光传感器应用于人血中IgG的检测,结果令人满意.
Using bio-compatible chitosan-nanogold composite film for protein immobilization on glassy carbon electrode,a novel electrochemiluminescent immunosensor with good conductivity for light and electricity was fabricated for human IgG detection.The composite film was characterized by scanning electron micrograph and electrochemical impedance spectrum.Based on a competitive format,an immunoassay method for human IgG was proposed using goat anti-human IgG labled with Ru（bpy）32＋ as tracer and novel co-reactant 2-（dibutylamino）ethanol for signal amplifying.Human IgG can be detected over a range of 20 ng mL-1-1.0 μg mL-1 with a detection limit of 6.5 ng mL-1.Acceptable results for determination of human IgG in human blood were obtained using the proposed electrochemiluminescent immunosensor.
出处
《中国科学：化学》
CSCD
北大核心
2010年第6期704-710,共7页
SCIENTIA SINICA Chimica
基金
国家自然科学基金(20805036)
高等学校博士学科点专项科研基金(200806351006)
重庆市自然科学基金(CSTC,2009BB5116)
西南大学基本科研业务费专项资金资助项目(XDJK2009B014)资助
