摘要
目的:探讨HLA-DQA1-DQB1连锁基因单倍体与成人缓慢进展型1型糖尿病(SPIDDM)和速发型1型糖尿病(FPIDDM)的相关性。方法:采用PCR/SSP技术检测本组1型糖尿病中102例SPIDDM患者和130例FPIDDM患者频率。结果:①HLA-DQA1*0301-DQB1*0201和DQA1*0501-DQB1*0201连锁基因单倍体与SPIDDM(Pc<0.001)和FPIDDM(Pc<0.001)均呈显著正相关。②HLA-DQA1*0301-DQB1*0301和DQA1*0301-DQB1*0602连锁基因单倍体与SPIDDM呈显著正相关(Pc<0.001)。③HLA-DQA1*0301-DQB1*0302、DQA1*0301-DQB1*0303及DRB1*0301-DQA1*0301-DQB1*0201连锁基因单倍体与FPIDDM呈显著正相关(均Pc<0.05);DQA1*0102等位基因中SPIDDM组16例(15.69%);FPIDDM组10例(7.69%)(P<0.05);DQA1*03基因SPIDDM组47例(46.08%),FPIDDM组79例(60.77%)(P<0.05);DQB1*0601基因SPIDDM组10例(9.8%),FPIDDM组4例(3.08%)(P<0.01)。结论:SPIDDM和FPIDDM虽然均为自身免疫性糖尿病,但其HLA表型并不完全相同,不同的HLA表型可能是决定患者起病方式及病情发展不同的因素之一。
Objective：To investigate the relevance of HLA-DQA1-DQB1 linked gene haploid with the slow progress adult type 1 diabetes mellitus（SPIDDM） and fast progressive type 1 diabetes mellitus（FPIDDM）.Methods：HLA-DR-DQ gene haplotypes in 102 SPIDDM patients and 130 FPIDDM patients were determined by PCR/SSP techniques.Results：①The frequencies of HLA-DQA1＊0301-DQB1＊0201 and DQA1＊0501-DQB1＊0201 gene haplotypes were significantly higher in both SPIDDM（Pc0.001） and FPIDDM（Pc0.001） patients.②The frequencies of HLA-DQA1＊0301-DQB1＊0301 and DQA1＊0301-DQB1＊0602 gene haplotypes were significantly higher in SPIDDM patients（Pc0.001）.③The frequencies of HLA-DQA1＊0301-DQB1＊0302,DQA1＊0301-DQB1＊0303 and DRB1＊0301-DQA1＊0301-DQB1＊0201 gene haplotypes were significantly higher in FPIDDM patients（all Pc0.05）.DQA1＊0102 allele 16 cases in SPIDDM group,accounting for 15.69%,10 cases in FPIDDM group,accounting for 7.69%（P0.05）;DQA1＊03 gene SPIDDM group of 47 patients（46.08%）,FPIDDM group of 79 patients（60.77%）（P0.05）;DQB1＊0601 gene SPIDDM group of 10 cases（9.8%）,FPIDDM group of 4 cases（3.08%）（P0.01）.Conclusion：Though SPIDDM and FPIDDM are both autoimmune diabetes,but its HLA phenotype is not the same,and different HLA phenotype may be one of the factors which determine the mode of onset and progression of different ways.
出处
《中国医药导报》
CAS
2011年第15期19-21,共3页
China Medical Herald
