摘要
以蔗糖、葡萄糖、可溶性淀粉为３种不同碳源，以Ｂ５培养基为基础，研究在不同碳源情况下，悬浮培养玫瑰茄细胞的生长，以及培养过程中细胞的氧消耗速率，同时也考察了在整个细胞生长过程中，辅酶Ｑ的含量变化。结果表明，蔗糖和葡萄糖能有效支持玫瑰茄细胞的生长，其细胞活力也高，表现在呼吸强度较高；可溶性淀粉不能被植物细胞利用，不仅表现在细胞生物量低，而且细胞活力差。
Based on B 5 medium, sucrose, glucose and soluble starch were used as carbon sources to study the growth state of suspension Roselle cell The oxygen respiration and coenzyme Q content were also studied through whole culture time The results showed that sucrose and glucose were good carbon sources which could support the growth of Roselle cell, and the cells had high respiration rate showed that the cell had high viability But the soluble starch couldn't support the growth of Roselle cell, and the cell with low viability
出处
《广西植物》
CAS
CSCD
北大核心
1999年第2期150-153,共4页
Guihaia
关键词
碳源
悬浮培养
玫瑰茄
呼吸强度
辅酶Q
Carbon source
suspension culture
Roselle
respiration rate
coenzyme Q
