摘要
以悬浮培养玫瑰茄细胞为研究对象 ,探讨了不同初始接种量以及蔗糖浓度对玫瑰茄细胞生长的影响 结果表明随着接种量增大 ,细胞生长启动加快 ,但细胞比生长速率却逐渐降低 ,这表明细胞的分裂启动存在着密度依赖现象 ,而细胞的生长却存在密度抑制现象 ;在 ρ(蔗糖 )为 2 0～ 5 0g/L范围内 ,当 ρ(蔗糖 )为 40g/L时 ,蔗糖是作为限制性基质而存在 ,而当 ρ(蔗糖 )超过 40g/L时 ,蔗糖就表现出基质抑制效应 ,这明显体现在玫瑰茄细胞对残糖消耗。
The suspension roselle cell was used to study the effects of inoculum amount and sucrose concentration on its growth. The results were as follows: the bigger the inoculum amount was, the faster the roselle cell started to grow, but the smaller the specific growth rate was. From these facts, the density dependent of cell starting to grow and density inhibited of cell growth were proposed; Sucrose was limiting substrate from 2% to 4%; But from 4% to higher concentration, the sucrose showed inhibition effect on cell growth. The dual effects of sucrose on cell growth were apparently seen from the utilization of nutrients ,such as sugar and nitrate.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2000年第4期51-54,共4页
Journal of South China Agricultural University
关键词
接种量
蔗糖浓度
悬浮培养
玫瑰茄
细胞培养
inoculum amount
sucrose concentration
suspension culture
roselle celx
