摘要
本文通过改变培养基中的氮源组成，来研究氮源的变化对悬浮培养玫瑰茄细胞的生长及硝态氮同化指征（活体内硝酸还原酶活力）的影响。实验表明，仅在含ＮＯ－３的培养基中能检测到硝酸还原酶活力，而仅含ＮＨ＋４的配方不能检测到该酶活力，表明硝酸还原酶是底物诱导酶。还探讨了培养基中不同的氮源组成对细胞生物量及ｐＨ的影响。
The growth and nitrate assimilation of suspension roselle cell were studied through changing the nitrogen source of B5mediumThe nitrate assimilatory ability of suspension roselle cell was represented by nitrate reductase activity in vivoThe nitrate reductase activity could only be detected in the medium which had nitrate in it, but in the medium which used ammonium as only nitrogen source, the nitrate reductase activity could not be detectedSo the nitrate reductase was verified again as substrate-inducing enzyme in cell culturing levelThe nitrogen source also had obvious effects on the biomass and pH of the medium.
出处
《广西植物》
CAS
CSCD
北大核心
1998年第2期169-172,共4页
Guihaia
关键词
氮源
悬浮培养
玫瑰茄
硝酸还原酶
Nitrogen source
suspension culture
roselle
nitrate reductase
