摘要
目的：建立一个快速生长的岩黄连悬浮细胞培养体系。方法：研究了接种量、基本培养基、初始pH值、不同碳源对岩黄连悬浮细胞生长的影响。结果：合适的接种量是7.5-10%（FW）,接种量过少会抑制细胞生长;B5和MS基本培养基均适合岩黄连细胞的生长;最佳初始培养基pH值为6.0,此时获得的细胞生物量最高;岩黄连悬浮细胞培养的生长周期为24d,最大生物量出现在第18d,达到14.1g/l（DW）;蔗糖比葡萄糖更有利于岩黄连细胞的生长,添加60g/l蔗糖所获得的生物量最高,达到18.5g/l（DW）。
Objective：Cell suspension cultures were established through selection of inoculum size. Methods：Basal medium, initial pH value, and carbon resource. Results：An inoculum size of 7.5 - 10% （FW） was suitable for cell growth and small inoculum size could impress biomass accumulation. The B5 and MS basal medium were fit to cell growth, and the favorable initial pH value of medium was 6. 0. The growth period of C. saxicola cell cultures was around 24d with highest biomass on day 18. Sucrose was much better than glucose for cell growth; the addition of 60g/l sucrose produced the most biomass with 18.5g/l （DW）.
出处
《生物技术》
CAS
CSCD
2007年第3期56-59,共4页
Biotechnology
关键词
悬浮细胞培养
岩黄连
接种量
cell suspension culture
Corydalis saxicola Bunting
inoculum size
