摘要
应用自动控制发酵设备，首先进行分批发酵试验摸索了地衣芽孢杆菌２７０９生长与代谢的基本规律。然后采用补料分批发酵方法限制生长基质浓度，测定了一系列（Ｓｉ，μｉ）、（μｊ，ｑｐｊ）数据，获得ＫＳ、μｍａｘ、α、β等参数的值，并且推导出了细胞生长与产物合成的动力学公式，从而证明了用Ｍｏｎｏｄ方程描述地衣芽孢杆菌２７０９生长速率与基质浓度关系的合理性和合成碱性蛋白酶的发酵属于生长部分关联型。
With autocontrol fermentation equipment,the basic law of growth and metabolism of Bacillus licheniformis 2709 was studied with the batch culture.Then the exact and enough points of (S, μ ) and ( μ ,q p )were obtained by controlling the concentration of the growth limiting substrate with the fed batch culture.The parameter values of K S,μ max ,α and β were acquired and the kinetic formula on cell growth rate and product synthesis rate were inferred.Thereby,the Monod formula was proved to be rational to describe the relationship between the cell growth rate of B.licheniformis 2709 and the concentration of the growth limiting substrate in the broth;And the data showed the fermentation type is one partially related to growth.
出处
《生物工程学报》
CAS
CSCD
北大核心
1998年第4期395-400,共6页
Chinese Journal of Biotechnology
