摘要
从１１份土样中分离出１０９株具碱性蛋白酶活力菌株。从中筛选出一株枯草芽孢杆菌（ＢａｃｉｌｌｕｓＳｕｂｔｉｌｉｓ）１０９号具高温碱性蛋白酶（ＴＡＰ）活力，在ｐＨ１０环境中好氧生长，最适生长温度为３７℃左右，ＴＡＰ活力平均为８６．９Ｕ／ｍＬ。１０９号菌株经亚硝基胍诱变后，得到一株ＴＡＰ活力提高至３０１Ｕ／ｍＬ的突变株Ｍ９２，该诱变株再用利福平抗性平皿自然分离得到一株具利福平抗性菌株Ｒ０１，其ＴＡＰ活力达３５０Ｕ／ｍＬ。
strain of bacteria with the activity of TAP were isolated. A mutant strainR01 with high yield of TAP and stability was obtained from wild Bacillus subtilis 109 bytreatment of 78 mg/L NTG and 0. 3 ￣ 0. 4 mg/L Rifampen. The enzyme activity Increased from 86. 9 U/mL to 350. 2 U/ml- after heat treatment at 70'C for 30 min.
出处
《清华大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1994年第3期83-87,共5页
Journal of Tsinghua University(Science and Technology)
关键词
高温
碱性
蛋白酶
枯草芽孢杆菌
thermotolerant alkaliphilic protease
Bacillus subtilis
isolation and mutation
