摘要
通过对培养基、水份含量、氮源、培养时间、培养基的起始PH以及培养温度的研究，测定TrichodermareeseiGAB纤维素酶的C_1酶和Cx酶的酶活，找到了一个最佳的条件，即：稻草粉：花生壳=4：1，物料：水份=2：3，以NH_4Cl、（NH_4）_2SO_4、NH_4H_2PO_4为氮源，起始pH为自然pH（约5．8），在28℃下培养84～96h，其酶活性为最高，达到C_1酶为58．2μ，Cx酶为1500μ。同时，也找到了减少生产成本的一条途径。
The factors including media, warer contents, nitrogen sources, cultural tine, originalpH and cultural temperatures were studied for producing cellulase by Trichoderma reesei GAB,The results showed that the best condition are f the ratio of material and water equals 2:3, thebest nitrogen sources are (NH4)2SO4, NH4Cl and NH4H2PO4, the original pH is natural pH, andthe cultural bine is 84-96 hours, the cultural temperature is at 28℃. The results showed thatcultured at that condition the cellulase C1 is 58.2μ and the cellulase Cx is 1500μ.
出处
《微生物学通报》
CAS
CSCD
北大核心
1998年第2期77-79,共3页
Microbiology China
基金
广东省科委科技攻关项目
关键词
纤维素酶
里氏木霉
Cellulase C1、Cx, Trichoderma reesei GAB, Enzyme activity
