摘要
从碱性土样中,分离到产碱性纤维素酶的细菌134株。经摇瓶初筛、复筛后,得到一株芽孢杆菌074,在pH9条件下能产生具有较高活性的纤维素酶。该菌株的最适生长pH为中性,最适生长温度为32℃。NaC1对酶的产生影响较大。羧甲基纤维素钠(CMC-Na)对酶的产生有一定促进作用,但不是唯一碳源。用最佳培养基和培养条件,48小时酶活性最高可达6u/ml。
A cellulolytic alkaline, Bacillus sp. 074, which can produce relative high alkaline carboxymethyl cellulase (CMCase) over pH 9.0, was isolated from soil. The optimum pH and temperature for growth of this strain were neutral and 32 C respectively. The existence of NaCl in the medium can affect the synthesis of the enzyme efficiently. However, the effect of carboxymethyl cellolose sodium on the enzyme was only a little, and it was not the only carbon sourse with which the strain can synthesize the enzyme. Under the optimization condition of culture, the enzyme activity can reach 6 unit/ml within 48 hours.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第1期38-44,共7页
Acta Microbiologica Sinica
