摘要
目的:筛选出能高效降解南瓜纤维素的菌株,以制备南瓜可溶性膳食纤维。方法:从土壤、腐烂的树叶和水果上分离出具有降解南瓜纤维素的菌株,用刚果红染色透明水解圈进行初筛,然后用CMC-Na、滤纸和南瓜渣为碳源测所有菌株及混合菌株的羧甲基纤维素酶活力,最终选出1株酶活力较高菌株进行下一步实验。结果:共分离到能够有效降解纤维素的共有5株细菌和7株真菌,通过测单菌株和混合菌株的酶活力,表明混合菌株的酶活力最大值比单菌株的酶活力最大值要高3倍之多。结论:混合菌株的酶活力比单株菌酶活力值高,发酵培养72h南瓜渣可溶性多糖降解力最强。
The objective of this article is to select the bacterial strains, which is capable of degrading pumpkin cellulose effectively, The experimental method is to extract the strains of degraded pumpkin cellulose from the soil, rotten leaves and fruit. From the beginning, transparent Congo red hydrolysis circles with the function of dyeing are operated to carry out the first selection, and next, CMC-Na, filter paper, pumpkin dregs are utilized to test for carbon source the enzymatic activity of carboxymethylcellulose from all the single and mixed bacterial strains. Ultimately, the strains with the strongest activity are taken as the representative to implement the activities on the next stage. The achievement of this experiment is to have chosen out five bacterial and seven fungi with the effective capacity of degrading cellulose. By means of the measurement of the enzymatic activity in the single and mixed strains, a result has been achieved that the enzymatic activity in mixed strains high three times than that of the single ones. Consequently, the conclusion is that the enzymatic activity in mixedstrains is much stronger than that of the single ones, fermentation cultivation 72 h pumpkin polysaccharide degradation soluble slag is the strongest force.
出处
《食品科技》
CAS
北大核心
2012年第3期2-5,15,共5页
Food Science and Technology
基金
国家自然科学基金项目(30972047)
"十二五"国家科技支撑计划项目(2011BAD23B01)
关键词
降解纤维素
分离
酶活力
cellulose degradation
depart
enzyme activity
