摘要
用电化学的方法，研究环丙沙星（ＣＰＦＸ）及其镁、锰络合物与脱氧核糖核酸（ＤＮＡ）的相互作用及其极谱伏安行为。结果在０１ｍｏｌ·Ｌ－１ＮＨ３ＮＨ４Ｃｌ（ｐＨ９２）溶液中，环丙沙星可与ＤＮＡ作用，产生一新的极谱峰Ｅｐ＝－１７２Ｖ（ｖｓＡｇ／ＡｇＣｌ），在有Ｍｇ２＋或Ｍｎ２＋存在时则生成三元络合物，产生一电位更负的新峰，峰电位Ｅｐ＝－１７８Ｖ，提示Ｍｇ２＋或Ｍｎ２＋离子参与药物与ＤＮＡ的作用。对它们的还原峰性质研究表明，电极还原反应是完全不可逆的，电流具有吸附性。本文还探讨了电极还原机理，认为参与电极还原的是三元络合物中的环丙沙星分子，进一步推测ＣＰＦＸＭｇ是嵌入ＤＮＡ的双螺旋结构中。
Interactions between ciprofloxacin (CPFX), Mg 2+ , Mn 2+ and DNA, and their polarographic and voltammetric behaviour were studied. In 0 1 mol·L -1 NH 3 NH 4Cl buffer solution (pH 9 2), a new reduction peak was obtained by linear sweep voltammetry with E p=-1 72 V(vs Ag/AgCl) when adding DNA to CPFX solution, which implies binding of CPFX with DNA. In the presence of Mg 2+ or Mn 2+ , another new sensitive reduction peak, whose peak potential is more negative ( E p=-1 78 V), was obtained which suggested that Mg 2+ or Mn 2+ took part in the interaction between CPFX and DNA resulting in a ternary complex of CPFX Mg DNA. The peak current ( i p) is proportional to the concentration of DNA over the range of 1 18×10 -4 ～3 33×10 -4 mol·L -1 . In this paper, the properties of the peak current were studied in detail, the result showed that the electrode reaction was irreversible and the i p was influenced by adsorption. The electrode reaction mechanism was also probed into. The CPFX molecule in the complex was reduced on the electrode. Furthermore, CPFX Mg was shown ot be intercalated between the stacked base pairs of native DNA.
出处
《药学学报》
CAS
CSCD
北大核心
1997年第4期314-317,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
关键词
环丙沙星
镁络合物
锰络合物
DNA
电化学法
DNA
CPFX Mg DNA complex
CPFX Mn DNA complex
Polarography
Voltammetry
