摘要
This paper describes a new fluorescence probe technique for the determination ofDNA, which is based on a self-quenching course of high concentration of o-hydroxybenzoicacid or o-aminobenzoic acid. Studies involving calf thymus (CT) DNA, salmon (SM) DNAand herring sperm(HS) DNA revealed that the differential value of fluorescence intensity inthe presence and absence of nucleic acid was proportional to the concentration of nucleic overthe range of about 15ng/mL^6.0μg/mL.
This paper describes a new fluorescence probe technique for the determination ofDNA, which is based on a self-quenching course of high concentration of o-hydroxybenzoicacid or o-aminobenzoic acid. Studies involving calf thymus (CT) DNA, salmon (SM) DNAand herring sperm(HS) DNA revealed that the differential value of fluorescence intensity inthe presence and absence of nucleic acid was proportional to the concentration of nucleic overthe range of about 15ng/mL^6.0μg/mL.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1997年第9期1456-1458,共3页
Chemical Journal of Chinese Universities
基金
国家教育委员会博士点基金
