摘要
用盐酸阿霉素作为嵌入剂石墨电极伏安法识别测定ＤＮＡ片段的研究方禹之，刘盛辉，何品刚（华东师范大学化学系，上海，２０００６２）关键词ＤＮＡ，嵌入剂，盐酸阿霉素，伏安法核酸分子杂交技术是近年来发展起来的检测ＤＮＡ分子的一种重要手段，目前主要采用的放射性同...
A synthesized 75-mer single stranded DNA (ssDNA-1) was covalently immobilized on a oxidized graphite electrode surface that had been activated using EDC and NHS.The immobilized ssDNA-1hybridized with various concentrations of complementary ssDNA-2 in hybridization buffer containing DXH.The dsDNA/DXH system was formed on the electrode surface. The cathodic waves of the DXH bound to the dsDNA with cyclic voltammetry were used for the determination of ssDNA-2.The reduction peak currents of DXH were linearly related to the concentration of ssDNA-2 between 1×10-5 to 5×10-7 mg/mL.The determination demonstrated the method is practicable.
出处
《高等学校化学学报》
CSCD
北大核心
1996年第8期1222-1224,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
DNA
嵌入剂
盐酸阿霉素
伏安法
DNA
Intercalator
Doxorubicin hydrochloridum
Voltammetry
