摘要
利用荧光光谱法研究了环丙沙星与铜（Ⅱ）、真核生物ＤＮＡ之间二元、三元络合物的形成。结果表明，铜（Ⅱ）和ＤＮＡ均可使环丙沙星的荧光强度发生猝灭。在铜（Ⅱ）存在下，ＤＮＡ对环丙沙星的荧光猝灭作用显著增强。根据荧光强度变化计算了二元、三元体系的形成常数，由此进一步证实了环丙沙星。
Using the method of fluorescence spectrometry,the formation of binary complexes of CPFX Cu(Ⅱ) and CPFX eucaryote DNA,and ternary complexes of CPFX Cu(Ⅱ) eucaryote DNA was studied.The results show that the fluorescent intensity of CPFX can be quenched in the presence of Cu(Ⅱ) or DNA in CPFX system.In the presence of Cu(Ⅱ),the fuorescence quenching action of DNA on CPFX was enhanced strongly.Based on the fluorescent intensity,the formation constants of CPFX Cu(Ⅱ) and CPFX DNA in either the presence or absence were calculated,and the mechanism of interaction among CPFX,Cu(Ⅱ) and DNA was presented.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
1999年第4期572-574,共3页
Spectroscopy and Spectral Analysis
基金
山西省自然科学基金
关键词
配合物
三元
相互作用
环丙沙星
铜
DNA
Fluorescence method, Ciprofloxacin, Cupric ions, Eucaryote DNA, Binary complex, Ternary complex
