摘要
目的优选三叶委陵菜的渗漉提取工艺条件。方法分别用紫外分光光度法和高效液相色谱法测定样品中的鞣质和儿茶素含量,采用正交实验设计对提取的条件进行优选。结果以乙醇浓度60%、浸泡时间24 h、乙醇用量10倍、流速0.3 mL.min-1为渗漉提取条件效果最好。结论该含量测定方法对鞣质和儿茶素定量准确,优选工艺稳定可行。
Objective To optimize the technology for the extraction of the percolate of the potentila freyniana borum. Methods The content of the total tannin and catechin in the samples were determined with UV spectrophotometry and HPLC, respectively. An orthogonal experimental design was adopted to optimize the technology for the extraction of the percolate. Results The optimum conditions for the extraction technology were found to be ： the concentration of ethanol was 60%, the time for immersion of the drug was 24 hours,10 volumes of ethanol were used for the extraction of 1 volume of the drug and the flow rate was 0.3 mL·min^-1 Conclusion The method for the content determination was shown to be accurate in the quantitation of total tannins and catechin in the potentila freyniana borum , and the optimiazed technology was stable and feasible .
出处
《医药导报》
CAS
2007年第6期652-654,共3页
Herald of Medicine
